How to calculate limit of detection, limit of quantification and signal to noise ratio? Ammonium acetate is a salt, not a buffer! Add 50 mL water and 2 mL DEG to a 250 mL separatory fu… 3. But, it is good for you to know how to calculate, and to do your calculations alone, so you will always solve this in other similar situations with acids that are mixture in water. Ammonium acetate is an ammonium salt obtained by reaction of ammonia with acetic acid. To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Ammonium formate (2.7